First, just avoid her attacks while maintaining your distance away from her. Eventually she will start spinning a web ready to do a belly flop, or starts to spit webs from a gray sack. Use the Power Slash Brush on the wed she spins or the gray sack and she will fall down. She will have some hooks at the tip of her spinnerets. Use the blossoms around the stadium to do the Vine Brush and connect two vines to the end of the hooks. Her thorax will open up and eyeballs will appear. Use your Devout Beads to attack the eyeballs and damage her. Keep doing this to defeat Spider Queen.